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| Martins,Polyana K.; Jordão,Berenice Q.; Yamanaka,Naoki; Farias,José R.B.; Beneventi,Magda A.; Binneck,Eliseu; Fuganti,Renata; Stolf,Renata; Nepomuceno,Alexandre L.. |
| Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water-stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell division; Differential display; Gene expression; Real time PCR; Soybean; Water stress. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300019 |
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| Yamanaka,Naoki; Sato,Hiroyuki; Yang,Zhenyu; Xu,Dong He; Catelli,Lizandra Lucy; Binneck,Eliseu; Arias,Carlos Alberto Arrabal; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima. |
| An understanding of the relationship of geographically different soybean gene pools, based on selectively neutral DNA markers would be useful for the selection of divergent parental cultivars for use in breeding. We assessed the relationships of 194 Chinese, 59 Japanese, and 19 Brazilian soybean cultivars (n = 272) using 12 simple sequence repeat (SSR) markers. Quantification Theory III and clustering analyses showed that the Chinese and Japanese cultivars were genetically quite distant to each other but not independent, while Brazilian cultivars were distantly related to the cultivars from the other two countries and formed a cluster that was distant from the other two gene pool clusters. Our results indicated that the Brazilian soybean gene pool is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA marker; Genetic relationship; Genetic resources; Glycine max. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100016 |
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| Yamanaka,Naoki; Fuentes,Francisco Horacio; Gilli,Javier Ramon; Watanabe,Satoshi; Harada,Kyuya; Ban,Tomohiro; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima; Homma,Yoshihisa. |
| The objective of this work was to identify genomic regions that underlie resistance to Fusarium tucumaniae sp. nov., the causing agent of sudden death syndrome (SDS) in soybean in South America, using a population with a genetic background different from that previously reported for Fusarium virguliforme sp. nov. (F. solani f. sp. glycines), also responsible for SDS in soybean. Although major genes and quantitative trait loci (QTL) for SDS resistance have been identified, little is known about the same disease caused by Fusarium tucumaniae sp. nov., in South America. To identify genetic factors related to resistance to F. tucumaniae and DNA markers associated with them, a QTL analysis was performed using recombinant inbred lines. The map locations of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Disease index; Residual heterozygous line; SDS. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000900006 |
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| Stolf,Renata; Medri,Moacyr Eurípedes; Pimenta,José Antônio; Boeger,Maria Regina Torres; Dias,Jaqueline; Lemos,Noélle Giacomini; Oliveira,Maria Cristina Neves de; Brogin,Rodrigo Luís; Yamanaka,Naoki; Neumaier,Norman; Farias,José Renato Bouça; Nepomuceno,Alexandre Lima. |
| In a greenhouse experiment, morpho-anatomical and micromorphometrical analyses of two soybean cultivars, MG/BR46 (Conquista) and BR16-tolerant and sensitive to drought, respectively-were used to study their water-deficit-tolerance strategies. Drought treatments were applied at reproductive stages from R2 to R7, where evaluations were conducted at 30 days and 45 days after stress started, respectively. The total length of Conquista plants (shoot + root) was greater than of BR16 plants. Pod dry weight was adversely affected due to the lack of moisture, decreasing productivity even of Conquista plants. Both the cultivars had normal development of root hairs; however, it was observed a decrease in the cortex:central cylinder ratio in BR16 stressed for 30 days,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anatomy; Leguminous; Drought; Root. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600002 |
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